Dysbiosis of Gut Microbiota With Reduced Trimethylamine‐N‐Oxide Level in Patients With Large‐Artery Atherosclerotic Stroke or Transient Ischemic Attack
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- Jia Yin
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Shuo‐Xi Liao
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Yan He
- Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
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- Shan Wang
- Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
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- Geng‐Hong Xia
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Fei‐Tong Liu
- Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
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- Jia‐Jia Zhu
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Chao You
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Qiong Chen
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Liang Zhou
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Su‐Yue Pan
- Department of Neurology, NanFang Hospital, Southern Medical University, Guangzhou, China
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- Hong‐Wei Zhou
- State Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, China
説明
<jats:sec xml:lang="en"> <jats:title>Background</jats:title> <jats:p xml:lang="en"> Gut microbiota has been suggested to play a role in almost all major diseases including cardio‐ and cerebrovascular diseases. A possible mechanism is the transformation of dietary choline and <jats:sc>l</jats:sc> ‐carnitine into trimethylamine by gut bacteria. This metabolite is further oxidized into trimethylamine‐N‐oxide ( <jats:styled-content style="fixed-case">TMAO</jats:styled-content> ) in liver and promotes atherogenesis. Nevertheless, little is known about gut microbial diversity and blood <jats:styled-content style="fixed-case">TMAO</jats:styled-content> levels in stroke patients. </jats:p> </jats:sec> <jats:sec xml:lang="en"> <jats:title>Methods and Results</jats:title> <jats:p xml:lang="en"> We performed a case‐control study of patients with large‐artery atherosclerotic ischemic stroke and transient ischemic attack. <jats:styled-content style="fixed-case">TMAO</jats:styled-content> was determined with liquid chromatography tandem mass spectrometry. Gut microbiome was profiled using Illumina sequencing of the 16S <jats:styled-content style="fixed-case">rRNA</jats:styled-content> V4 tag. Within the asymptomatic control group, participants with and without carotid atherosclerotic plaques showed similar levels of <jats:styled-content style="fixed-case">TMAO</jats:styled-content> without a significant difference in gut microbiota; however, the gut microbiome of stroke and transient ischemic attack patients was clearly different from that of the asymptomatic group. Stroke and transient ischemic attack patients had more opportunistic pathogens, such as <jats:italic>Enterobacter</jats:italic> , <jats:italic>Megasphaera</jats:italic> , <jats:italic>Oscillibacter</jats:italic> , and <jats:italic>Desulfovibrio</jats:italic> , and fewer commensal or beneficial genera including <jats:italic>Bacteroides</jats:italic> , <jats:italic>Prevotella</jats:italic> , and <jats:italic>Faecalibacterium</jats:italic> . This dysbiosis was correlated with the severity of the disease. The <jats:styled-content style="fixed-case">TMAO</jats:styled-content> level in the stroke and transient ischemic attack patients was significantly lower, rather than higher, than that of the asymptomatic group. </jats:p> </jats:sec> <jats:sec xml:lang="en"> <jats:title>Conclusions</jats:title> <jats:p xml:lang="en"> Participants with asymptomatic atherosclerosis did not exhibit an obvious change in gut microbiota and blood <jats:styled-content style="fixed-case">TMAO</jats:styled-content> levels; however, stroke and transient ischemic attack patients showed significant dysbiosis of the gut microbiota, and their blood <jats:styled-content style="fixed-case">TMAO</jats:styled-content> levels were decreased. </jats:p> </jats:sec>
収録刊行物
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- Journal of the American Heart Association
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Journal of the American Heart Association 4 (11), e002699-, 2015-10-29
Ovid Technologies (Wolters Kluwer Health)