-
- Luisa Barzon
- Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, I-35121 Padova, Italy
-
- Enrico Lavezzo
- Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, I-35121 Padova, Italy
-
- Valentina Militello
- Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, I-35121 Padova, Italy
-
- Stefano Toppo
- Department of Biological Chemistry, University of Padova, I-35121 Padova, Italy
-
- Giorgio Palù
- Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, I-35121 Padova, Italy
書誌事項
- 公開日
- 2011-11-14
- 権利情報
-
- https://creativecommons.org/licenses/by/3.0/
- DOI
-
- 10.3390/ijms12117861
- 公開者
- MDPI AG
説明
<jats:p>Novel DNA sequencing techniques, referred to as “next-generation” sequencing (NGS), provide high speed and throughput that can produce an enormous volume of sequences with many possible applications in research and diagnostic settings. In this article, we provide an overview of the many applications of NGS in diagnostic virology. NGS techniques have been used for high-throughput whole viral genome sequencing, such as sequencing of new influenza viruses, for detection of viral genome variability and evolution within the host, such as investigation of human immunodeficiency virus and human hepatitis C virus quasispecies, and monitoring of low-abundance antiviral drug-resistance mutations. NGS techniques have been applied to metagenomics-based strategies for the detection of unexpected disease-associated viruses and for the discovery of novel human viruses, including cancer-related viruses. Finally, the human virome in healthy and disease conditions has been described by NGS-based metagenomics.</jats:p>
収録刊行物
-
- International Journal of Molecular Sciences
-
International Journal of Molecular Sciences 12 (11), 7861-7884, 2011-11-14
MDPI AG