Establishment of mouse expanded potential stem cells
書誌事項
- 公開日
- 2017-10
- 権利情報
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- http://www.springer.com/tdm
- DOI
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- 10.1038/nature24052
- 10.17863/cam.13696
- 公開者
- Springer Science and Business Media LLC
この論文をさがす
説明
Mouse embryonic stem cells derived from the epiblast1 contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm2 upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.
収録刊行物
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- Nature
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Nature 550 (7676), 393-397, 2017-10
Springer Science and Business Media LLC
