Mouse β-Defensin 3 Is an Inducible Antimicrobial Peptide Expressed in the Epithelia of Multiple Organs
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- Robert Bals
- <!--label omitted: 1-->Institute for Human Gene Therapy, Department of Medicine and Molecular and Cellular Engineering, The Wistar Institute,1 and
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- Xiaorong Wang
- <!--label omitted: 1-->Institute for Human Gene Therapy, Department of Medicine and Molecular and Cellular Engineering, The Wistar Institute,1 and
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- Rupalie L. Meegalla
- <!--label omitted: 1-->Institute for Human Gene Therapy, Department of Medicine and Molecular and Cellular Engineering, The Wistar Institute,1 and
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- Sigrid Wattler
- <!--label omitted: 2-->Lexicon Genetics Incorporated, The Woodlands, Texas 773812
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- Daniel J. Weiner
- <!--label omitted: 1-->Institute for Human Gene Therapy, Department of Medicine and Molecular and Cellular Engineering, The Wistar Institute,1 and
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- Michael C. Nehls
- <!--label omitted: 2-->Lexicon Genetics Incorporated, The Woodlands, Texas 773812
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- James M. Wilson
- <!--label omitted: 1-->Institute for Human Gene Therapy, Department of Medicine and Molecular and Cellular Engineering, The Wistar Institute,1 and
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- J. R. McGhee
- editor
抄録
<jats:title>ABSTRACT</jats:title> <jats:p> One component of host defense at mucosal surfaces is epithelium-derived peptides with antimicrobial activity called defensins. We describe in this report the isolation and characterization of a murine homologue of human β-defensin 2 (hBD-2) called mouse β-defensin 3 (mBD-3). The predicted amino acid sequence shows the hallmark features of other known epithelial defensins, including the ordered array of six cysteine residues. Analysis of a genomic clone of mBD-3 revealed two exons separated by a 1.7-kb intron. The mBD-3 gene is localized at the proximal portion of chromosome 8, the site where genes for mouse α- and β-defensins are found. Under basal condition, mBD-3 transcripts were detected at low levels in epithelial cells of surface organs, such as the intestine and lung. After instillation of <jats:italic>Pseudomonas aeruginosa</jats:italic> PAO1 into mouse airways, mBD-3-specific mRNA was upregulated significantly not only in large airways but also in the small bowel and liver. Recombinant mBD-3 peptide, produced from a baculovirus expression system, showed antimicrobial activity against <jats:italic>P. aeruginosa</jats:italic> PAO1 (MIC of 8 μg/ml) and <jats:italic>Escherichia coli</jats:italic> D31 (MIC of 16 μg/ml) in a salt-dependent manner. This study demonstrates that a murine homologue of hBD-2 is present in the respiratory system and other mucosal surfaces. These similarities between murine and human host defense apparatus provide further impetus to evaluate the mouse as a model for studying the human innate host defense system. </jats:p>
収録刊行物
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- Infection and Immunity
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Infection and Immunity 67 (7), 3542-3547, 1999-07
American Society for Microbiology
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詳細情報 詳細情報について
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- CRID
- 1362544419199767168
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- ISSN
- 10985522
- 00199567
- http://id.crossref.org/issn/00199567
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- データソース種別
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- Crossref