Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches
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- Oleksii Nevskyi
- Institute of Physical Chemistry RWTH Aachen University Landoltweg 2 52074 Aachen Germany
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- Dmytro Sysoiev
- Department of Chemistry University of Konstanz Universitätsstrasse 10 78464 Konstanz Germany
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- Alex Oppermann
- Institute of Physical Chemistry RWTH Aachen University Landoltweg 2 52074 Aachen Germany
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- Thomas Huhn
- Department of Chemistry University of Konstanz Universitätsstrasse 10 78464 Konstanz Germany
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- Dominik Wöll
- Institute of Physical Chemistry RWTH Aachen University Landoltweg 2 52074 Aachen Germany
書誌事項
- 公開日
- 2016-09-13
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/anie.201606791
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>The in situ imaging of soft matter is of paramount importance for a detailed understanding of functionality on the nanoscopic scale. Although super‐resolution fluorescence microscopy methods with their unprecedented imaging capabilities have revolutionized research in the life sciences, this potential has been far less exploited in materials science. One of the main obstacles for a more universal application of super‐resolved fluorescence microscopy methods is the limitation of readily available suitable dyes to overcome the diffraction limit. Here, we report a novel diarylethene‐based photoswitch with a highly fluorescent closed and a nonfluorescent open form. Its photophysical properties, switching behavior, and high photostability make the dye an ideal candidate for photoactivation localization microscopy (PALM). It is capable of resolving apolar structures with an accuracy far beyond the diffraction limit of optical light in cylindrical micelles formed by amphiphilic block copolymers.</jats:p>
収録刊行物
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 55 (41), 12698-12702, 2016-09-13
Wiley