Dengue Virus Infection of Mast Cells Triggers Endothelial Cell Activation

  • Michael G. Brown
    Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
  • Laura L. Hermann
    Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
  • Andrew C. Issekutz
    Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
  • Jean S. Marshall
    Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
  • Derek Rowter
    Pediatrics, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
  • Ayham Al-Afif
    Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
  • Robert Anderson
    Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada

書誌事項

公開日
2011-01-15
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/jvi.01630-10
公開者
American Society for Microbiology

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説明

<jats:title>ABSTRACT</jats:title><jats:p>Vascular perturbation is a hallmark of severe forms of dengue disease. We show here that antibody-enhanced dengue virus infection of primary human cord blood-derived mast cells (CBMCs) and the human mast cell-like line HMC-1 results in the release of factor(s) which activate human endothelial cells, as evidenced by increased expression of the adhesion molecules ICAM-1 and VCAM-1. Endothelial cell activation was prevented by pretreatment of mast cell-derived supernatants with a tumor necrosis factor (TNF)-specific blocking antibody, thus identifying TNF as the endothelial cell-activating factor. Our findings suggest that mast cells may represent an important source of TNF, promoting vascular endothelial perturbation following antibody-enhanced dengue virus infection.</jats:p>

収録刊行物

  • Journal of Virology

    Journal of Virology 85 (2), 1145-1150, 2011-01-15

    American Society for Microbiology

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