Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression

DOI Web Site 被引用文献15件
  • Fei Song
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
  • Joseph F. Smith
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
  • Makoto T. Kimura
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
  • Arlene D. Morrow
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
  • Tomoki Matsuyama
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
  • Hiroki Nagase
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
  • William A. Held
    Departments of Molecular and Cellular Biology and Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263; Plant Functions Laboratory, Computational Science Division, Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan

説明

<jats:p> Early studies proposed that DNA methylation could have a role in regulating gene expression during development [Riggs, A.D. (1975) <jats:italic>Cytogenet. Cell Genet.</jats:italic> 14, 9–25]. However, some studies of DNA methylation in known tissue-specific genes during development do not support a major role for DNA methylation. In the results presented here, tissue-specific differentially methylated regions (TDMs) were first identified, and then expression of genes associated with these regions correlated with methylation status. Restriction landmark genomic scanning (RLGS) was used in conjunction with virtual RLGS to identify 150 TDMs [Matsuyama, T., Kimura, M.T., Koike, K., Abe, T., Nakao, T., Asami, T., Ebisuzaki, T., Held, W.A., Yoshida, S. & Nagase, H. (2003) <jats:italic>Nucleic Acids Res.</jats:italic> 31, 4490–4496]. Analysis of 14 TDMs by methylation-specific PCR and by bisulfite genomic sequencing confirms that the regions identified by RLGS are differentially methylated in a tissue-specific manner. The results indicate that 5% or more of the CpG islands are TDMs, disputing the general notion that all CpG islands are unmethylated. Some of the TDMs are within 5′ promoter CpG islands of genes, which exhibit a tissue-specific expression pattern that is consistent with methylation status and a role in tissue differentiation. </jats:p>

収録刊行物

被引用文献 (15)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ