Expression of Human Gaucher Disease Gene GBA Generates Neurodevelopmental Defects and ER Stress in Drosophila Eye
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- Andrea Dardis
- editor
書誌事項
- 公開日
- 2013-08-02
- 権利情報
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- http://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.1371/journal.pone.0069147
- 公開者
- Public Library of Science (PLoS)
説明
Gaucher disease (GD) is the most common of the lysosomal storage disorders and is caused by defects in the GBA gene encoding glucocerebrosidase (GlcCerase). The accumulation of its substrate, glucocylceramide (GlcCer) is considered the main cause of GD. We found here that the expression of human mutated GlcCerase gene (hGBA) that is associated with neuronopathy in GD patients causes neurodevelopmental defects in Drosophila eyes. The data indicate that endoplasmic reticulum (ER) stress was elevated in Drosophila eye carrying mutated hGBAs by using of the ER stress markers dXBP1 and dBiP. We also found that Ambroxol, a potential pharmacological chaperone for mutated hGBAs, can alleviate the neuronopathic phenotype through reducing ER stress. We demonstrate a novel mechanism of neurodevelopmental defects mediated by ER stress through expression of mutants of human GBA gene in the eye of Drosophila.
収録刊行物
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- PLoS ONE
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PLoS ONE 8 (8), e69147-, 2013-08-02
Public Library of Science (PLoS)
- Tweet
キーワード
- Science
- Developmental Disabilities
- Blotting, Western
- Molecular Sequence Data
- Eye
- Real-Time Polymerase Chain Reaction
- Nervous System
- Animals, Genetically Modified
- Animals
- Humans
- Amino Acid Sequence
- RNA, Messenger
- Cells, Cultured
- Gaucher Disease
- Reverse Transcriptase Polymerase Chain Reaction
- Q
- R
- Endoplasmic Reticulum Stress
- Disease Models, Animal
- Drosophila melanogaster
- Mutation
- Medicine
- Glucosylceramidase
- Research Article
