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- Angela Kwok Fung Lo
- *Viral Oncology Program, Sidney Kimmel Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21231;
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- Ka Fai To
- Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China,
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- Kwok Wai Lo
- Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China,
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- Raymond Wai Ming Lung
- Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China,
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- Jan Wai Ying Hui
- Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China,
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- Gangling Liao
- *Viral Oncology Program, Sidney Kimmel Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21231;
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- S. Diane Hayward
- *Viral Oncology Program, Sidney Kimmel Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21231;
書誌事項
- 公開日
- 2007-10-09
- DOI
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- 10.1073/pnas.0702896104
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p>Epstein–Barr virus (EBV) was the first human virus found to encode microRNAs (miRNAs), but the function of these miRNAs has been obscure. Nasopharyngeal carcinoma (NPC) is associated with EBV infection, and the EBV-encoded LMP1 is believed to be a key factor in NPC development. However, detection of LMP1 protein in NPC is variable. Here, we report that EBV-encoded BART miRNAs target the 3′ UTR of the LMP1 gene and negatively regulate LMP1 protein expression. These miRNAs also modulate LMP1-induced NF-κB signaling and alleviate the cisplatin sensitivity of LMP1-expressing NPC cells. Consistent with a previous study on the NPC C666-1 cell line and C15 xenograft, we found abundant expression of BART miRNAs in NPC tissues. Furthermore, DNA sequencing revealed that the 3′ UTR of LMP1 is highly conserved in NPC-derived EBV isolates. The data provide insight into the discrepancy between LMP1 transcript and protein detection in NPC and highlight the role of the EBV miRNAs in regulating LMP1 downstream signaling to promote cancer development.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 104 (41), 16164-16169, 2007-10-09
Proceedings of the National Academy of Sciences