Leveraging single-cell genomics to expand the fungal tree of life

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Description

<jats:title>Abstract</jats:title><jats:p>Environmental DNA surveys reveal that most fungal diversity represents uncultured species. We sequenced the genomes of eight uncultured species across the fungal tree of life using a new single-cell genomics pipeline. We show that, despite a large variation in genome and gene space recovery from each single amplified genome (SAG), ≥90% can be recovered by combining multiple SAGs. SAGs provide robust placement for early-diverging lineages and infer a diploid ancestor of fungi. Early-diverging fungi share metabolic deficiencies and show unique gene expansions correlated with parasitism and unculturability. Single-cell genomics holds great promise in exploring fungal diversity, life cycles and metabolic potential.</jats:p>

Journal

  • Nature Microbiology

    Nature Microbiology 3 (12), 1417-1428, 2018-10-08

    Springer Science and Business Media LLC

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