<i>Arabidopsis</i> primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body
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- Liang Song
- Biology Department and Huck Institute of the Life Sciences, Pennsylvania State University, University Park, PA 16802;
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- Meng-Hsuan Han
- Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208-3500; and
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- Joanna Lesicka
- Department of Gene Expression, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland
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- Nina Fedoroff
- Biology Department and Huck Institute of the Life Sciences, Pennsylvania State University, University Park, PA 16802;
書誌事項
- 公開日
- 2007-03-27
- DOI
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- 10.1073/pnas.0701061104
- 公開者
- Proceedings of the National Academy of Sciences
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説明
<jats:p> Small regulatory microRNAs (miRNAs) are encoded in long precursors and are released from them during processing by cleavage within partially duplexed stem–loop structures. In the present work we investigated the role of the <jats:italic>Arabidopsis</jats:italic> nuclear RNA-binding protein HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pri-miR171a). The <jats:italic>miR171a</jats:italic> gene is complex, with multiple transcription start sites, as well as alternative splicing of exons and alternative polyadenylation sites. Both HYL1 and DCL1 proteins are required for processing of the major pri-miR171a, spliced and polyadenylated forms of which accumulate in plants homozygous for mutations in either gene, but not in wild-type plants. In transiently transfected <jats:italic>Arabidopsis</jats:italic> protoplasts, HYL1-mCherry and YFP-DCL1 fusion proteins colocalize to small nuclear bodies similar to Cajal bodies but lacking the Cajal body marker Atcoilin. The HYL1 protein coimmunoprecipitates with miR171a and miR159a precursors, indicating that it is an integral component of the precursor processing machinery. Thus, the distinct HYL1- and DCL1-containing nuclear bodies may be miRNA precursor processing sites. Alternatively, they may be assembly and storage sites for the miRNA precursor processing machinery. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 104 (13), 5437-5442, 2007-03-27
Proceedings of the National Academy of Sciences