Pressure—A Gateway to Fundamental Insights into Protein Solvation, Dynamics, and Function

  • Trung Quan Luong
    Department of Chemistry and Chemical Biology, Physical Chemistry TU Dortmund University, Dortmund Otto-Hahn-Str. 6 d-44221 Dortmund Germany
  • Shobhna Kapoor
    Department of Chemistry and Chemical Biology, Physical Chemistry TU Dortmund University, Dortmund Otto-Hahn-Str. 6 d-44221 Dortmund Germany
  • Roland Winter
    Department of Chemistry and Chemical Biology, Physical Chemistry TU Dortmund University, Dortmund Otto-Hahn-Str. 6 d-44221 Dortmund Germany

書誌事項

公開日
2015-10-27
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/cphc.201500669
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>Now that the centennial anniversary of the first report on pressure denaturation of proteins by Nobel Laureate P. W. Bridgman can be celebrated, this Review on the application of high pressure as a key variable for studying the energetics and interactions of proteins appears. We demonstrate that combined temperature–pressure‐dependent studies help delineate the free‐energy landscape of proteins and elucidate which features are essential in determining their stability. Pressure perturbation also serves as an important tool to explore fluctuations in proteins and reveal their conformational substates. From shaping the free‐energy landscape of proteins themselves to that of their interactions, conformational fluctuations not only dictate a plethora of biological processes, but are also implicated in a number of debilitating diseases. Finally, the advantages of using pressure to explore biomolecular assemblies and modulate enzymatic reactions are discussed.</jats:p>

収録刊行物

  • ChemPhysChem

    ChemPhysChem 16 (17), 3555-3571, 2015-10-27

    Wiley

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