Simple Microplate Method for Determination of Urinary Iodine

  • Toshinori Ohashi
    Pharmaceutical Research Laboratory, Hitachi Chemical Co., Ltd., 13-1, Higashi-cho 4-chome, Hitachi-shi, Ibaraki-ken 317-8555, Japan
  • Mitsuo Yamaki
    Pharmaceutical Research Laboratory, Hitachi Chemical Co., Ltd., 13-1, Higashi-cho 4-chome, Hitachi-shi, Ibaraki-ken 317-8555, Japan
  • Chandrakant S Pandav
    International Council for Control of Iodine Deficiency Disorders, New Delhi 110029, India
  • Madhu G Karmarkar
    International Council for Control of Iodine Deficiency Disorders, New Delhi 110029, India
  • Minoru Irie
    International Council for Control of Iodine Deficiency Disorders, Tokyo 112-0001, Japan

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<jats:title>Abstract</jats:title> <jats:p>Background: Urinary iodine is a good biochemical marker for control of iodine deficiency disorders. Our aim was to develop and validate a simple, rapid, and quantitative method based on the Sandell–Kolthoff reaction, incorporating both the reaction and the digestion process into a microplate format.</jats:p> <jats:p>Methods: Using a specially designed sealing cassette to prevent loss of vapor and cross-contamination among wells, ammonium persulfate digestion was performed in a microplate in an oven at 110 °C for 60 min. After the digestion mixture was transferred to a transparent microplate and the Sandell–Kolthoff reaction was performed at 25 °C for 30 min, urinary iodine was measured by a microplate reader at 405 nm.</jats:p> <jats:p>Results: The mean recovery of iodine added to urine was 98% (range, 89–109%). The theoretical detection limit, defined as 2 SD from the zero calibrator, was 0.11 μmol/L (14 μg/L iodine). The mean intra- and interassay CVs for samples with iodine concentrations of 0.30–3.15 μmol/L were ≤10%. The new method agreed well with the conventional chloric acid digestion method (n = 70; r = 0.991; y = 0.944x + 0.04; Sy|x = 0.10) and with the inductively coupled plasma mass spectrometry method (n = 61; r = 0.979; y = 0.962x + 0.03; Sy|x = 0.20). The agreement was confirmed by difference plots. The distributions of iodine concentrations for samples from endemic areas of iodine deficiency diseases showed similar patterns among the above three methods.</jats:p> <jats:p>Conclusions: Our new method, incorporating the whole process into a microplate format, is readily applicable and allows rapid monitoring of urinary iodine.</jats:p>

収録刊行物

  • Clinical Chemistry

    Clinical Chemistry 46 (4), 529-536, 2000-04-01

    Oxford University Press (OUP)

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