Distinct regulation of <i>CAB</i> and <i>PHYB</i> gene expression by similar circadian clocks
抄録
<jats:title>Summary</jats:title><jats:p>Phytochrome B (phyB) is a major phytochrome active in light‐grown plants. The circadian clock controls the expression of the <jats:italic>PHYB</jats:italic> gene. We have used the luciferase reporter gene (<jats:italic>LUC</jats:italic>) to monitor the rhythmic expression of <jats:italic>PHYB</jats:italic> in photoreceptor and clock‐associated mutant backgrounds. Surprisingly, we found that <jats:italic>PHYB</jats:italic> and <jats:italic>CAB</jats:italic> expression have different free‐running periods, indicating that separate circadian clocks control these genes. The effects of mutations show that the clocks share common components. This suggests that they are copies of the same clock mechanism in different locations, most likely in different cell layers. Furthermore, we show that phyB is required for a negative feedback loop that strongly antagonises the expression of <jats:italic>PHYB</jats:italic>. Compared to a system with only one clock, this regulatory complexity might allow the phase of peak expression for one clock‐controlled gene to alter, relative to other genes or to changing environmental conditions.</jats:p>
収録刊行物
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- The Plant Journal
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The Plant Journal 32 (4), 529-537, 2002-11
Wiley