A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects
-
- Alexandre Pfister
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Marie Barberon
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Julien Alassimone
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Lothar Kalmbach
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Yuree Lee
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Joop EM Vermeer
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Misako Yamazaki
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Guowei Li
- Biochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, France
-
- Christophe Maurel
- Biochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, France
-
- Junpei Takano
- Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
-
- Takehiro Kamiya
- Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United Kingdom
-
- David E Salt
- Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United Kingdom
-
- Daniele Roppolo
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
-
- Niko Geldner
- Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
抄録
<jats:p>The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)—major players of endodermal differentiation—into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.</jats:p>
収録刊行物
-
- eLife
-
eLife 3 e03115-, 2014-09-16
eLife Sciences Publications, Ltd