Studies on the role of the 2'-5'-oligoadenylate synthetase-RNase L pathway in beta interferon-mediated inhibition of encephalomyocarditis virus replication
-
- R Kumar
- Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
-
- D Choubey
- Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
-
- P Lengyel
- Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
-
- G C Sen
- Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
書誌事項
- 公開日
- 1988-09
- 権利情報
-
- https://journals.asm.org/non-commercial-tdm-license
- DOI
-
- 10.1128/jvi.62.9.3175-3181.1988
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:p>Interferons inhibit the replication of vesicular stomatitis virus (VSV), but not of encephalomyocarditis virus (EMCV), in mouse JLSV-11 cells. We report the isolation of clonal derivatives from this cell line in which the replication of both viruses is impaired by interferons. These clones were selected from the parental line by virtue of their rescue by interferon treatment from the cytopathic effects of EMCV infection. In one such clone, RK8, the replication of VSV and EMCV and the production of resident murine leukemia virus were inhibited by interferon. On the other hand, in clone RK6, which was isolated without any selection, the replication of VSV, but not of EMCV, was impaired by interferons. The levels of 2'-5'-oligoadenylate synthetase mRNA and enzyme activity were similarly elevated upon interferon treatment in the two clones. However, the level of RNase L, as determined by binding and cross-linking of a radiolabeled 2'-5'-oligoadenylate derivative, was much lower in RK6 cells than in RK8 cells. In accord with this observation, the introduction of 2'-5'-oligoadenylates into cells inhibited protein synthesis much less strongly in RK6 cells than in RK8 cells. These results are consistent with the notion that the 2'-5'-oligoadenylate-dependent RNase L may be a mediator of the inhibition of EMCV replication by interferons.</jats:p>
収録刊行物
-
- Journal of Virology
-
Journal of Virology 62 (9), 3175-3181, 1988-09
American Society for Microbiology
