Identification of <i>Candida</i> species in the clinical laboratory: a review of conventional, commercial, and molecular techniques
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- KH Neppelenbroek
- Department of Prosthodontics Bauru Dental School University of São Paulo‐USP Bauru São Paulo Brazil
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- RS Seó
- Department of Prosthodontics Varzea Grande School of Dentistry Varzea Grande Mato Grosso Brazil
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- VM Urban
- Department of Dentistry Ponta Grossa State University‐UEPG Ponta Grossa Paraná Brazil
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- S Silva
- Department of Biological Engineering Institute for Biotechnology and Bioengineering University of Minho Braga Portugal
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- LN Dovigo
- Department of Social Dentistry Araraquara Dental School UNESP – Univ. Estadual Paulista Araraquara São Paulo Brazil
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- JH Jorge
- Department of Dental Materials and Prosthodontics Araraquara Dental School UNESP – Univ. Estadual Paulista Araraquara São Paulo Brazil
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- NH Campanha
- Department of Dentistry Ponta Grossa State University‐UEPG Ponta Grossa Paraná Brazil
説明
<jats:p>In healthy individuals, <jats:italic>Candida</jats:italic> species are considered commensal yeasts of the oral cavity. However, these microorganisms can also act as opportunist pathogens, particularly the so‐called non‐<jats:italic>albicans Candida</jats:italic> species that are increasingly recognized as important agents of human infection. Several surveys have documented increased rates of <jats:italic>C. glabrata</jats:italic>,<jats:italic> C. tropicalis</jats:italic>,<jats:italic> C. guilliermondii</jats:italic>,<jats:italic> C. dubliniensis</jats:italic>,<jats:italic> C. parapsilosis</jats:italic>, and <jats:italic>C. krusei</jats:italic> in local and systemic fungal infections. Some of these species are resistant to antifungal agents. Consequently, rapid and correct identification of species can play an important role in the management of candidiasis. Conventional methods for identification of <jats:italic>Candida</jats:italic> species are based on morphological and physiological attributes. However, accurate identification of all isolates from clinical samples is often complex and time‐consuming. Hence, several manual and automated rapid commercial systems for identifying these organisms have been developed, some of which may have significant sensitivity issues. To overcome these limitations, newer molecular typing techniques have been developed that allow accurate and rapid identification of <jats:italic>Candida</jats:italic> species. This study reviewed the current state of identification methods for yeasts, particularly <jats:italic>Candida</jats:italic> species.</jats:p>
収録刊行物
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- Oral Diseases
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Oral Diseases 20 (4), 329-344, 2013-05-16
Wiley