Cloning and characterization of the goadsporin biosynthetic gene cluster from Streptomyces sp. TP-A0584
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- Hiroyasu Onaka
- Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
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- Mizuho Nakaho
- Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
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- Keiko Hayashi
- Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
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- Yasuhiro Igarashi
- Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
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- Tamotsu Furumai
- Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
書誌事項
- 公開日
- 2005-12-01
- DOI
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- 10.1099/mic.0.28420-0
- 公開者
- Microbiology Society
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説明
<jats:p>The biosynthetic gene cluster of goadsporin, a polypeptide antibiotic containing thiazole and oxazole rings, was cloned from <jats:italic>Streptomyces</jats:italic> sp. TP-A0584. The cluster contains a structural gene, <jats:italic>godA</jats:italic>, and nine <jats:italic>god</jats:italic> (<jats:underline>go</jats:underline>a<jats:underline>d</jats:underline>sporin) genes involved in post-translational modification, immunity and transcriptional regulation. Although the gene organization is similar to typical bacteriocin biosynthetic gene clusters, each goadsporin biosynthetic gene shows low homology to these genes. Goadsporin biosynthesis is initiated by the translation of <jats:italic>godA</jats:italic>, and the subsequent cyclization, dehydration and acetylation are probably catalysed by <jats:italic>godD</jats:italic>, <jats:italic>godE</jats:italic>, <jats:italic>godF</jats:italic>, <jats:italic>godG</jats:italic> and <jats:italic>godH</jats:italic> gene products. <jats:italic>godI</jats:italic> shows high similarity to the 54 kDa subunit of the signal recognition particle and plays an important role in goadsporin immunity. Furthermore, four goadsporin analogues were produced by site-directed mutagenesis of <jats:italic>godA</jats:italic>, suggesting that this biosynthesis machinery is used for the heterocyclization of peptides.</jats:p>
収録刊行物
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- Microbiology
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Microbiology 151 (12), 3923-3933, 2005-12-01
Microbiology Society