-
- Gregory Thyssen
- Waksman Institute, Rutgers University, Piscataway, NJ 08854; and
-
- Zora Svab
- Waksman Institute, Rutgers University, Piscataway, NJ 08854; and
-
- Pal Maliga
- Waksman Institute, Rutgers University, Piscataway, NJ 08854; and
書誌事項
- 公開日
- 2012-01-30
- DOI
-
- 10.1073/pnas.1114297109
- 公開者
- National Academy of Sciences
この論文をさがす
説明
<jats:p> Our objective was to test whether or not plastids and mitochondria, the two DNA-containing organelles, move between cells in plants. As our experimental approach, we grafted two different species of tobacco, <jats:italic>Nicotiana tabacum</jats:italic> and <jats:italic>Nicotiana sylvestris</jats:italic> . Grafting triggers formation of new cell-to-cell contacts, creating an opportunity to detect cell-to-cell organelle movement between the genetically distinct plants. We initiated tissue culture from sliced graft junctions and selected for clonal lines in which gentamycin resistance encoded in the <jats:italic>N. tabacum</jats:italic> nucleus was combined with spectinomycin resistance encoded in <jats:italic>N. sylvestris</jats:italic> plastids. Here, we present evidence for cell-to-cell movement of the entire 161-kb plastid genome in these plants, most likely in intact plastids. We also found that the related mitochondria were absent, suggesting independent movement of the two DNA-containing organelles. Acquisition of plastids from neighboring cells provides a mechanism by which cells may be repopulated with functioning organelles. Our finding supports the universality of intercellular organelle trafficking and may enable development of future biotechnological applications. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 109 (7), 2439-2443, 2012-01-30
National Academy of Sciences
