RNA Tertiary Structure Mediation by Adenosine Platforms

DOI Web Site 5 Citations
  • Jamie H. Cate
    J. H. Cate, K. Zhou, J. A. Doudna, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.
  • Anne R. Gooding
    A. R. Gooding, E. Podell, B. L. Golden, A. A. Szewczak, T. R. Cech, Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309.
  • Elaine Podell
    A. R. Gooding, E. Podell, B. L. Golden, A. A. Szewczak, T. R. Cech, Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309.
  • Kaihong Zhou
    J. H. Cate, K. Zhou, J. A. Doudna, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.
  • Barbara L. Golden
    A. R. Gooding, E. Podell, B. L. Golden, A. A. Szewczak, T. R. Cech, Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309.
  • Alexander A. Szewczak
    A. R. Gooding, E. Podell, B. L. Golden, A. A. Szewczak, T. R. Cech, Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309.
  • Craig E. Kundrot
    C. E. Kundrot, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309.
  • Thomas R. Cech
    A. R. Gooding, E. Podell, B. L. Golden, A. A. Szewczak, T. R. Cech, Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309.
  • Jennifer A. Doudna
    J. H. Cate, K. Zhou, J. A. Doudna, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.

Description

<jats:p>The crystal structure of a group I intron domain reveals an unexpected motif that mediates both intra- and intermolecular interactions. At three separate locations in the 160-nucleotide domain, adjacent adenosines in the sequence lie side-by-side and form a pseudo-base pair within a helix. This adenosine platform opens the minor groove for base stacking or base pairing with nucleotides from a noncontiguous RNA strand. The platform motif has a distinctive chemical modification signature that may enable its detection in other structured RNAs. The ability of this motif to facilitate higher order folding provides one explanation for the abundance of adenosine residues in internal loops of many RNAs.</jats:p>

Journal

  • Science

    Science 273 (5282), 1696-1699, 1996-09-20

    American Association for the Advancement of Science (AAAS)

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