Genomic characterization of non-O1, non-O139 <i>Vibrio cholerae</i> reveals genes for a type III secretion system

  • Michelle Dziejman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Davide Serruto
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Vincent C. Tam
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Derek Sturtevant
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Pornphan Diraphat
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Shah M. Faruque
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • M. Hasibur Rahman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • John F. Heidelberg
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Jeremy Decker
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Li Li
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Kate T. Montgomery
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • George Grills
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • Raju Kucherlapati
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...
  • John J. Mekalanos
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115; Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh; The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Harvard Partners Genome Center, 65 Landsdowne Street, Cambridge, MA 02139; and Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, New Research Building, 77...

書誌事項

公開日
2005-02-22
DOI
  • 10.1073/pnas.0409918102
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p> Non-O1, non-O139 <jats:italic>Vibrio cholerae</jats:italic> can cause gastroenteritis and extraintestinal infections, but, unlike O1 and O139 strains of <jats:italic>V. cholerae</jats:italic> , little is known about the virulence gene content of non-O1, non-O139 strains and their phylogenetic relationship to other pathogenic <jats:italic>V. cholerae</jats:italic> . Comparative genomic microarray analysis of four pathogenic non-O1, non-O139 strains indicates that these strains are quite divergent from O1 and O139 strains. Genomic sequence analysis of a non-O1, non-O139 strain (AM-19226) that appeared particularly pathogenic in experimental animals suggests that this strain carries a type III secretion system (TTSS) that is related to the TTSS2 gene cluster found in a pandemic clone of <jats:italic>Vibrio parahaemolyticus</jats:italic> . The genes for this <jats:italic>V. cholerae</jats:italic> TTSS system appear to be present in many clinical and environmental non-O1, non-O139 strains, including at least one clone that is globally distributed. We hypothesize that the TTSS present in some pathogenic strains of non-O1, non-O139 <jats:italic>V. cholerae</jats:italic> may be involved in the virulence and environmental fitness of these strains. </jats:p>

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