Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

  • Paul D. Fraser
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Susanne Romer
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Cathie A. Shipton
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Philippa B. Mills
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Joy W. Kiano
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Norihiko Misawa
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Rachel G. Drake
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Wolfgang Schuch
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
  • Peter M. Bramley
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom

抄録

<jats:p> Phytoene synthase from the bacterium <jats:italic>Erwinia uredovora</jats:italic> ( <jats:italic>crt</jats:italic> B) has been overexpressed in tomato ( <jats:italic>Lycopersicon esculentum</jats:italic> Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary transformants (T <jats:sub>0</jats:sub> ) were 2–4-fold higher than the controls, whereas phytoene, lycopene, β-carotene, and lutein levels were increased 2.4-, 1.8-, and 2.2-fold, respectively. The biosynthetically related isoprenoids, tocopherols plastoquinone and ubiquinone, were unaffected by changes in carotenoid levels. The progeny (T <jats:sub>1</jats:sub> and T <jats:sub>2</jats:sub> generations) inherited both the transgene and phenotype. Determination of enzyme activity and Western blot analysis revealed that the CRTB protein was plastid-located and catalytically active, with 5–10-fold elevations in total phytoene synthase activity. Metabolic control analysis suggests that the presence of an additional phytoene synthase reduces the regulatory effect of this step over the carotenoid pathway. The activities of other enzymes in the pathway (isopentenyl diphosphate isomerase, geranylgeranyl diphosphate synthase, and incorporation of isopentenyl diphosphate into phytoene) were not significantly altered by the presence of the bacterial phytoene synthase. </jats:p>

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