Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner
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- Paul D. Fraser
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Susanne Romer
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Cathie A. Shipton
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Philippa B. Mills
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Joy W. Kiano
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Norihiko Misawa
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Rachel G. Drake
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Wolfgang Schuch
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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- Peter M. Bramley
- School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX, United Kingdom; Syngenta, Jealott's Hill Research Station, Bracknell, Berks RG42 6EY, United Kingdom; Kirin Brewery, Central Laboratories for Key Technology, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan; and Syngenta Wheat Improvement Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
抄録
<jats:p> Phytoene synthase from the bacterium <jats:italic>Erwinia uredovora</jats:italic> ( <jats:italic>crt</jats:italic> B) has been overexpressed in tomato ( <jats:italic>Lycopersicon esculentum</jats:italic> Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary transformants (T <jats:sub>0</jats:sub> ) were 2–4-fold higher than the controls, whereas phytoene, lycopene, β-carotene, and lutein levels were increased 2.4-, 1.8-, and 2.2-fold, respectively. The biosynthetically related isoprenoids, tocopherols plastoquinone and ubiquinone, were unaffected by changes in carotenoid levels. The progeny (T <jats:sub>1</jats:sub> and T <jats:sub>2</jats:sub> generations) inherited both the transgene and phenotype. Determination of enzyme activity and Western blot analysis revealed that the CRTB protein was plastid-located and catalytically active, with 5–10-fold elevations in total phytoene synthase activity. Metabolic control analysis suggests that the presence of an additional phytoene synthase reduces the regulatory effect of this step over the carotenoid pathway. The activities of other enzymes in the pathway (isopentenyl diphosphate isomerase, geranylgeranyl diphosphate synthase, and incorporation of isopentenyl diphosphate into phytoene) were not significantly altered by the presence of the bacterial phytoene synthase. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 99 (2), 1092-1097, 2002-01-22
Proceedings of the National Academy of Sciences