Application of magnetic chromatography to the isolation of lysosomes from fibroblasts of patients with lysosomal storage disorders
書誌事項
- 公開日
- 1998-12-28
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1016/s0014-5793(98)01578-6
- 公開者
- Wiley
この論文をさがす
説明
<jats:p>A method for the purification of lysosomes from fibroblasts has been developed which uses endocytosis of superparamagnetic colloidal iron dextran particles followed by separation of the iron‐containing lysosomes in a magnetic field. This permitted isolation of lysosomes from fibroblasts from patients with infantile sialic acid storage disorder and other lysosomal storage diseases in which a shift in lysosomal density induced by the storage material prevents purification by centrifugation in a Percoll gradient. The magnetic lysosomes isolated from these cells are very similar to those from normal cells as judged by lysosomal marker enzyme activity and 2D‐PAGE analysis of the enriched proteins.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 441 (3), 369-372, 1998-12-28
Wiley