Functional expression of a locust tyramine receptor in murine erythroleukaemia cells
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説明
<jats:title>Abstract</jats:title><jats:p>The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the <jats:italic>Locusta migratoria</jats:italic> tyramine receptor (Tyr<jats:italic>Loc</jats:italic>), an insect G protein‐coupled receptor. Functional agonist‐dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL‐Tyr<jats:italic>Loc</jats:italic>). Tyramine elicited a dose‐dependent increase of cytosolic Ca<jats:sup>2+</jats:sup>‐ions and an attenuation of forskolin‐induced cyclic adenosine monophosphate (AMP) production. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR/MEL expression system in functional assays for G protein‐coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways.</jats:p>
収録刊行物
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- Insect Molecular Biology
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Insect Molecular Biology 10 (6), 541-548, 2001-12
Wiley
