Comprehensive analysis of a dipeptide library to identify ghrelin release‐modulating peptides
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- Junya Nakato
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
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- Hayato Aoki
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
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- Yuki Tokuyama
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
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- Yuta Yamamoto
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
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- Hiroshi Iwakura
- The First Department of Medicine Wakayama Medical University Japan
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- Shigenobu Matsumura
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
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- Kazuo Inoue
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
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- Kousaku Ohinata
- Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University Uji Japan
書誌事項
- 公開日
- 2019-07-12
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/1873-3468.13522
- 公開者
- Wiley
この論文をさがす
説明
<jats:p>We performed a comprehensive analysis of ghrelin release‐modulating activity of a dipeptide library using MGN3‐1, a ghrelin‐producing cell line. We found that most dipeptides suppress ghrelin secretion, whereas the N‐terminal Ser‐containing dipeptides and a few others stimulate it. N‐terminal amino acid residues, but not C‐terminal residues, play a dominant role in the effects of dipeptides. Among dipeptides, Leu‐Ile (LI) and Ser‐Val (SV) most strongly suppress and stimulate ghrelin secretion, respectively. LI activates G<jats:sub>i</jats:sub> signaling and SV acts <jats:italic>via</jats:italic> the MAPK pathway. Orally administered LI and SV reduce and increase plasma ghrelin levels and food intake in mice, respectively. In conclusion, LI and SV, found based on the comprehensive screening of a dipeptide library, modulate ghrelin secretion <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic>.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 593 (18), 2637-2645, 2019-07-12
Wiley