Fractionation of Glycopeptides by Affinity Column Chromatography on Concanavalin A-Sepharose1
書誌事項
- 公開日
- 1975-10
- DOI
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- 10.1093/oxfordjournals.jbchem.a130956
- 公開者
- Oxford University Press (OUP)
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説明
Using [3H]-labeled oligosaccharides, we found that the presence of at least two alpha-mannosyl residues with free hydroxyl groups at C-3, 4, and 6 is required for oligosaccharides to be related by a concanavalin A-Sepharose column. This finding is also applicable to N-[14C]acetylated glycopeptides. Thus, the concanavalin A-Sepharose column might become a useful tool for structural studies of glycopeptides and oligosaccharides and for their fractionation. Glycopeptides prepared from the trypsinate of rat fibroblasts, which has been purified by paper electrophoresis, were further separated into two fractions by chromatography on a concanavalin A-Sepharose column.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 78 (4), 687-696, 1975-10
Oxford University Press (OUP)
