Human HL-60 myeloid leukemia cells transport dehydroascorbic acid via the glucose transporters and accumulate reduced ascorbic acid

DOI PDF 被引用文献5件
  • JC Vera
    Program in Molecular Pharmacology and Therapeutics, Memorial Sloan- Kettering Cancer Center, New York, NY 10021.
  • CI Rivas
    Program in Molecular Pharmacology and Therapeutics, Memorial Sloan- Kettering Cancer Center, New York, NY 10021.
  • RH Zhang
    Program in Molecular Pharmacology and Therapeutics, Memorial Sloan- Kettering Cancer Center, New York, NY 10021.
  • CM Farber
    Program in Molecular Pharmacology and Therapeutics, Memorial Sloan- Kettering Cancer Center, New York, NY 10021.
  • DW Golde
    Program in Molecular Pharmacology and Therapeutics, Memorial Sloan- Kettering Cancer Center, New York, NY 10021.

説明

<jats:title>Abstract</jats:title> <jats:p>The cellular accumulation of vitamin C, a substance critical to human physiology, is mediated by transporters located at the cell membrane, and is regulated in a cell-specific manner. Neoplastic cells may have special needs for vitamin C. Therefore, we investigated the transport of vitamin C in a human myeloid leukemia cell line (HL-60). The HL-60 cells lacked the capacity to transport the reduced form of vitamin C, ascorbic acid, but they showed a remarkable ability to transport the oxidized form of vitamin C, dehydroascorbic acid (DHA). Uptake- accumulation studies indicated that the HL-60 cells accumulated ascorbic acid when provided with DHA. Kinetic analysis showed the presence of two functional activities involved in the uptake of DHA, one with low affinity and one with high affinity. Cytochalasin B and phloretin, which inhibit the passage of glucose through the facilitative glucose transporters, also inhibited the transport of DHA by HL-60 cells. Transport of DHA was completed by D- but not L-hexoses, and was sensitive to D-hexose-dependent counter transport acceleration. These data support the concept that HL-60 myeloid leukemic cells transport DHA through the facilitative hexose transporters (glucose transporters) and accumulate the reduced form of ascorbic acid.</jats:p>

収録刊行物

  • Blood

    Blood 84 (5), 1628-1634, 1994-09-01

    American Society of Hematology

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