Importance of the Converter Region for the Motility of Myosin as Revealed by the Studies on Chimeric Chara Myosins
書誌事項
- 公開日
- 2004-11
- 権利情報
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- https://www.elsevier.com/tdm/userlicense/1.0/
- DOI
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- 10.1016/j.jmb.2004.09.039
- 公開者
- Elsevier BV
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説明
A long alpha-helix in myosin head constitutes a lever arm together with light chains. It is known from X-ray crystallographic studies that the first three turns of this lever arm alpha-helix are inserted into the converter region of myosin. We previously showed that chimeric Chara myosin in which the motor domain of Chara myosin was connected to the lever arm alpha-helix of Dictyostelium myosin had motility far less than that expected for the motor domain of Chara myosin. Here, we replaced the inserted three turns of alpha-helix of Dictyostelium myosin with that of the Chara myosin and found that the replacement enhanced the motility 2.6-fold without changing the ATPase activity so much. The result clearly showed the importance of interaction between the converter region and the lever arm alpha-helix for the efficient motility of myosin.
収録刊行物
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- Journal of Molecular Biology
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Journal of Molecular Biology 344 (2), 311-315, 2004-11
Elsevier BV