Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.
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- D K Palmer
- Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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- K O'Day
- Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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- H L Trong
- Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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- H Charbonneau
- Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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- R L Margolis
- Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
書誌事項
- 公開日
- 1991-05
- DOI
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- 10.1073/pnas.88.9.3734
- 公開者
- Proceedings of the National Academy of Sciences
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説明
<jats:p>CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 88 (9), 3734-3738, 1991-05
Proceedings of the National Academy of Sciences