The bHLH Transcription Factor POPEYE Regulates Response to Iron Deficiency in<i>Arabidopsis</i>Roots

DOI PDF 24 Citations
  • Terri A. Long
    Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, North Carolina 27708
  • Hironaka Tsukagoshi
    Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, North Carolina 27708
  • Wolfgang Busch
    Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, North Carolina 27708
  • Brett Lahner
    Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907
  • David E. Salt
    Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907
  • Philip N. Benfey
    Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, North Carolina 27708

Description

<jats:title>Abstract</jats:title><jats:p>Global population increases and climate change underscore the need for better comprehension of how plants acquire and process nutrients such as iron. Using cell type–specific transcriptional profiling, we identified a pericycle-specific iron deficiency response and a bHLH transcription factor, POPEYE (PYE), that may play an important role in this response. Functional analysis of PYE suggests that it positively regulates growth and development under iron-deficient conditions. Chromatin immunoprecipitation-on-chip analysis and transcriptional profiling reveal that PYE helps maintain iron homeostasis by regulating the expression of known iron homeostasis genes and other genes involved in transcription, development, and stress response. PYE interacts with PYE homologs, including IAA–Leu Resistant3 (ILR3), another bHLH transcription factor that is involved in metal ion homeostasis. Moreover, ILR3 interacts with a third protein, BRUTUS (BTS), a putative E3 ligase protein, with metal ion binding and DNA binding domains, which negatively regulates the response to iron deficiency. PYE and BTS expression is also tightly coregulated. We propose that interactions among PYE, PYE homologs, and BTS are important for maintaining iron homeostasis under low iron conditions.</jats:p>

Journal

  • The Plant Cell

    The Plant Cell 22 (7), 2219-2236, 2010-07-01

    Oxford University Press (OUP)

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