Selective Killing of Breast Cancer Cells by Doxorubicin‐Loaded Fluorescent Gold Nanoclusters: Confocal Microscopy and FRET

  • Shyamtanu Chattoraj
    Department of Physical Chemistry Indian Association for the Cultivation of Science, Jadavpur Kolkata 700 032 India), Fax
  • Asif Amin
    Department of Physical Chemistry Indian Association for the Cultivation of Science, Jadavpur Kolkata 700 032 India), Fax
  • Batakrishna Jana
    Organic & Medicinal Chemistry Division CSIR-Indian Institute of Chemical Biology, Jadavpur Kolkata 700 032 India
  • Saswat Mohapatra
    Organic & Medicinal Chemistry Division CSIR-Indian Institute of Chemical Biology, Jadavpur Kolkata 700 032 India
  • Surajit Ghosh
    Organic & Medicinal Chemistry Division CSIR-Indian Institute of Chemical Biology, Jadavpur Kolkata 700 032 India
  • Kankan Bhattacharyya
    Department of Physical Chemistry Indian Association for the Cultivation of Science, Jadavpur Kolkata 700 032 India), Fax

書誌事項

公開日
2015-12-04
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/cphc.201500982
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>Fluorescent gold nanoclusters (AuNCs) capped with lysozymes are used to deliver the anticancer drug doxorubicin to cancer and noncancer cells. Doxorubicin‐loaded AuNCs cause the highly selective and efficient killing (90 %) of breast cancer cells (MCF7) (IC<jats:sub>50</jats:sub>=155 n<jats:sc>m</jats:sc>). In contrast, the killing of the noncancer breast cells (MCF10A) by doxorubicin‐loaded AuNCs is only 40 % (IC<jats:sub>50</jats:sub>=4500 n<jats:sc>m</jats:sc>). By using a confocal microscope, the fluorescence spectrum and decay of the AuNCs were recorded inside the cell. The fluorescence maxima (at ≈490–515 nm) and lifetime (≈2 ns), of the AuNCs inside the cells correspond to Au<jats:sub>10–13</jats:sub>. The intracellular release of doxorubicin from AuNCs is monitored by Förster resonance energy transfer (FRET) imaging.</jats:p>

収録刊行物

  • ChemPhysChem

    ChemPhysChem 17 (2), 253-259, 2015-12-04

    Wiley

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