Aldosterone induces circadian gene expression of clock genes in H9c2 cardiomyoblasts

DOI PDF Web Site Web Site PubMed 被引用文献3件

書誌事項

公開日
2007-07-20
権利情報
  • http://www.springer.com/tdm
DOI
  • 10.1007/s00380-006-0968-3
公開者
Springer Science and Business Media LLC

この論文をさがす

説明

We examined mRNA expression of the clock genes (Per1, Per2, and Bmal1) and PAI-1 (plasminogen activator inhibitor-1) after aldosterone treatment every 4 h up to 48 h in H9c2 cardiomyoblasts by reverse transcription-polymerase chain reaction. To block the MR (mineralocorticoid receptor), the MR antagonist, spironolactone, was added to the medium 1 h before aldosterone treatment. Aldosterone induced an initial increase and rhythmic expression of Per1, while spironolactone attenuated the acute increase in Per1 mRNA induced by aldosterone. On the other hand, aldosterone did not increase the Per2 mRNA in the acute phase, but thereafter induced a rhythmic expression of Per2. Aldosterone also induced rhythmic expression of Bmal1, a positive element of the clock genes. The rhythm of Bmal1 mRNA was anti-phase of that of Per2 mRNA. Aldosterone induced an acute increase in PAI-1 mRNA, but did not induce rhythmic expression of PAI-1. The present study demonstrated first that aldosterone regulates expression of the clock genes Per1, Per2, and Bmal1, and increases PAI-1 expression in H9c2 cardiomyoblasts. Second, an acute increase in Per1 mRNA after aldosterone treatment is mediated through MR. Third, clock genes are not related to PAI-1 expression in H9c2 cardiomyoblasts.

収録刊行物

  • Heart and Vessels

    Heart and Vessels 22 (4), 254-260, 2007-07-20

    Springer Science and Business Media LLC

被引用文献 (3)*注記

もっと見る

キーワード

問題の指摘

ページトップへ