Supramolecular Hemoprotein Linear Assembly by Successive Interprotein Heme−Heme Pocket Interactions
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- Hiroaki Kitagishi
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
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- Koji Oohora
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
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- Hiroyasu Yamaguchi
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
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- Hideaki Sato
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
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- Takashi Matsuo
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
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- Akira Harada
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
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- Takashi Hayashi
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan, Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan, and Department of Medical Biochemistry, Kurume University School of Medicine, Kurume 830-0011, Japan
書誌事項
- 公開日
- 2007-08-01
- DOI
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- 10.1021/ja073295q
- 公開者
- American Chemical Society (ACS)
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説明
We demonstrate a new strategy for the construction of supramolecular hemoprotein assemblies. A synthetic heme was selectively introduced onto the surface Cys residue of the cytochrome b562 single mutant (H63C) through a thioether bond. After removal of the native heme of the H63C mutant by acid denaturation followed by neutralization, the externally attached heme on the apoprotein surface was inserted into the vacant heme pocket of the other apoprotein. Therefore, the interprotein heme−heme pocket interaction produces a unique submicrometer-sized linear hemoprotein fiber, determined by size exclusion chromatography and atomic force microscopy. This methodology should be widely applicable to the creation of new nanobiomaterials based on a functional hemoprotein.
収録刊行物
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- Journal of the American Chemical Society
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Journal of the American Chemical Society 129 (34), 10326-10327, 2007-08-01
American Chemical Society (ACS)