Enzymatic activity on a chip: The critical role of protein orientation

DOI Web Site Web Site 被引用文献9件

書誌事項

公開日
2005-02
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/pmic.200400948
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>We compare the catalytic activities of enzymes immobilized on silicon surfaces with and without orientation. While oriented sulfotransferases selectively immobilized on an otherwise zero‐background surface <jats:italic>via</jats:italic> 6×His tags faithfully reflect activities of solution phase enzymes, those with random orientation on the surface do not. This finding demonstrates that controlling the orientation of immobilized protein molecules and designing an ideal local chemical environment on the solid surface are both essential if protein microarrays are to be used as quantitative tools in biomedical research.</jats:p>

収録刊行物

  • PROTEOMICS

    PROTEOMICS 5 (2), 416-419, 2005-02

    Wiley

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