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- Steven B. Smith
- Department of Genetics, University of Washington, Seattle, WA 98195.
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- Paul K. Aldridge
- Center for Process Analytical Chemistry, Department of Chemistry BG-10, University of Washington, Seattle, WA 98195.
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- James B. Callis
- Center for Process Analytical Chemistry, Department of Chemistry BG-10, University of Washington, Seattle, WA 98195.
書誌事項
- 公開日
- 1989-01-13
- DOI
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- 10.1126/science.2911733
- 公開者
- American Association for the Advancement of Science (AAAS)
この論文をさがす
説明
<jats:p>Individual DNA molecules undergoing agarose gel electrophoresis were viewed with the aid of a fluorescence microscope. Molecular shape and orientation were studied in both steady and pulsed electric fields. It was observed that (i) DNA macromolecules advanced lengthwise through the gel in an extended configuration, (ii) the molecules alternately contracted and lengthened as they moved, (iii) the molecules often became hooked around obstacles in a U-shape for extended periods, and (iv) the molecules displayed elasticity as they extended from both ends at once. A computer model has been developed that simulates the migration of the molecules in a rotating-field gel electrophoresis experiment.</jats:p>
収録刊行物
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- Science
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Science 243 (4888), 203-206, 1989-01-13
American Association for the Advancement of Science (AAAS)