Reciprocal Responses in the Interaction between Arabidopsis and the Cell-Content-Feeding Chelicerate Herbivore Spider Mite

  • Vladimir Zhurov
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Marie Navarro
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Kristie A. Bruinsma
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Vicent Arbona
    Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, E-12071 Castelló de la Plana, Spain (V.A., A.G.-C.);
  • M. Estrella Santamaria
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Marc Cazaux
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Nicky Wybouw
    Department of Crop Protection, Faculty of Bioscience Engineering (N.W., T.V.L.) and
  • Edward J. Osborne
    Department of Biology (E.J.O., R.M.C.) and Center for Cell and Genome Science (R.M.C.), University of Utah, Salt Lake City, Utah 84112;
  • Cherise Ens
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Cristina Rioja
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Vanessa Vermeirssen
    Department of Plant Biotechnology and Bioinformatics (V.V., Y.V.d.P.), Ghent University, B–9000 Ghent, Belgium;
  • Ignacio Rubio-Somoza
    Max Planck Institute for Developmental Biology, D–72076 Tuebingen, Germany (I.R.-S., M.S.);
  • Priti Krishna
    School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia (P.K.); and
  • Isabel Diaz
    Centro de Biotecnologıa y Genomica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28223 Madrid, Spain (M.E.S., I.D.);
  • Markus Schmid
    Max Planck Institute for Developmental Biology, D–72076 Tuebingen, Germany (I.R.-S., M.S.);
  • Aurelio Gómez-Cadenas
    Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, E-12071 Castelló de la Plana, Spain (V.A., A.G.-C.);
  • Yves Van de Peer
    Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B–9052 Ghent, Belgium (Y.V.d.P.);
  • Miodrag Grbić
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);
  • Richard M. Clark
    Department of Biology (E.J.O., R.M.C.) and Center for Cell and Genome Science (R.M.C.), University of Utah, Salt Lake City, Utah 84112;
  • Thomas Van Leeuwen
    Department of Crop Protection, Faculty of Bioscience Engineering (N.W., T.V.L.) and
  • Vojislava Grbić
    Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7 (V.Z., M.N., K.A.B., M.E.S., M.C., C.E., C.R., P.K., M.G., V.G.);

抄録

<jats:title>Abstract</jats:title><jats:p>Most molecular-genetic studies of plant defense responses to arthropod herbivores have focused on insects. However, plant-feeding mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g. lepidopteran larvae or aphids). The two-spotted spider mite (Tetranychus urticae) is among the most significant mite pests in agriculture, feeding on a staggering number of plant hosts. To understand the interactions between spider mite and a plant at the molecular level, we examined reciprocal genome-wide responses of mites and its host Arabidopsis (Arabidopsis thaliana). Despite differences in feeding guilds, we found that transcriptional responses of Arabidopsis to mite herbivory resembled those observed for lepidopteran herbivores. Mutant analysis of induced plant defense pathways showed functionally that only a subset of induced programs, including jasmonic acid signaling and biosynthesis of indole glucosinolates, are central to Arabidopsis’s defense to mite herbivory. On the herbivore side, indole glucosinolates dramatically increased mite mortality and development times. We identified an indole glucosinolate dose-dependent increase in the number of differentially expressed mite genes belonging to pathways associated with detoxification of xenobiotics. This demonstrates that spider mite is sensitive to Arabidopsis defenses that have also been associated with the deterrence of insect herbivores that are very distantly related to chelicerates. Our findings provide molecular insights into the nature of, and response to, herbivory for a representative of a major class of arthropod herbivores.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 164 (1), 384-399, 2013-11-27

    Oxford University Press (OUP)

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