Somatic Embryogenesis in Sawara Cypress (<i>Chamaecyparis pisifera</i>Sieb. et Zucc.) for Stable and Efficient Plant Regeneration, Propagation and Protoplast Culture

DOI Web Site 被引用文献5件
  • Emilio Maruyama
    Transformation Laboratory, Department of Molecular & Cell Biology, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan.
  • Yoshihisa Hosoi
    Transformation Laboratory, Department of Molecular & Cell Biology, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan.
  • Katsuaki Ishii
    Transformation Laboratory, Department of Molecular & Cell Biology, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan.

書誌事項

公開日
2002-02
DOI
  • 10.1007/bf02762595
公開者
Informa UK Limited

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説明

Somatic embryogenesis inChamaecyparis pisifera was initiated from immature seeds collected from the end of June to early July. We obtained initiation frequencies ranging from 12.5 to 33.3% using whole seed explants in liquid media. Embryogenic cultures were maintained and proliferated for more than a year in solid and liquid media. High maturation frequencies of ‘high quality’ embryos were obtained on maturation media containing abscisic acid (ABA), activated charcoal (AC), and polyethylene glycol (PEG) as osmotic agent. More than one thousand cotyledonary embryos on average per 100 mg initial fresh weight of embryogenic cells were attained on medium containing 100µM ABA, 2 gL−1 AC, and 150 gL−1 PEG. About 97% germination frequencies and 92% plant conversion rates were achieved without any pretreatment. Growing of plants regenerated from somatic embryos has been monitored in the field. Furthermore, a procedure for culture of protoplasts isolated from embryonal masses was also described.

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