A digital 3D reference atlas reveals cellular growth patterns shaping the Arabidopsis ovule
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- Athul Vijayan
- Plant Developmental Biology, School of Life Sciences, Technical University of Munich, Freising, Germany
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- Rachele Tofanelli
- Plant Developmental Biology, School of Life Sciences, Technical University of Munich, Freising, Germany
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- Sören Strauss
- Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany
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- Lorenzo Cerrone
- Heidelberg Collaboratory for Image Processing, Dept. of Physics and Astronomy, Heidelberg University, Heidelberg, Germany
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- Adrian Wolny
- Heidelberg Collaboratory for Image Processing, Dept. of Physics and Astronomy, Heidelberg University, Heidelberg, Germany
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- Joanna Strohmeier
- Plant Developmental Biology, School of Life Sciences, Technical University of Munich, Freising, Germany
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- Anna Kreshuk
- European Molecular Biology Laboratory, Heidelberg, Germany
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- Fred A Hamprecht
- Heidelberg Collaboratory for Image Processing, Dept. of Physics and Astronomy, Heidelberg University, Heidelberg, Germany
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- Richard S Smith
- Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany
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- Kay Schneitz
- Plant Developmental Biology, School of Life Sciences, Technical University of Munich, Freising, Germany
説明
<jats:p>A fundamental question in biology is how morphogenesis integrates the multitude of processes that act at different scales, ranging from the molecular control of gene expression to cellular coordination in a tissue. Using machine-learning-based digital image analysis, we generated a three-dimensional atlas of ovule development in<jats:italic>Arabidopsis thaliana</jats:italic>, enabling the quantitative spatio-temporal analysis of cellular and gene expression patterns with cell and tissue resolution. We discovered novel morphological manifestations of ovule polarity, a new mode of cell layer formation, and previously unrecognized subepidermal cell populations that initiate ovule curvature. The data suggest an irregular cellular build-up of<jats:italic>WUSCHEL</jats:italic>expression in the primordium and new functions for<jats:italic>INNER NO OUTER</jats:italic>in restricting nucellar cell proliferation and the organization of the interior chalaza. Our work demonstrates the analytical power of a three-dimensional digital representation when studying the morphogenesis of an organ of complex architecture that eventually consists of 1900 cells.</jats:p>
収録刊行物
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- eLife
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eLife 10 e63262-, 2021-01-06
eLife Sciences Publications, Ltd
