Genetic polymorphisms and antiviral activity in the bovine <i>MX1</i> gene
抄録
<jats:title>Summary</jats:title><jats:p>Bovine <jats:italic>MX1</jats:italic> cDNAs consisting of 2280 bp from 11 animals of five breeds and from a cultured cell line were sequenced and compared with previously reported data. Ten nucleotide substitutions were synonymous mutations, and a single nucleotide substitution at 458 resulted in an amino acid exchange of Ile (ATT) and Met (ATG). A 13‐bp deletion–insertion mutation was also found in the 3′‐UTR. Based on the nucleotide substitutions found in this study, bovine <jats:italic>MX1</jats:italic> cDNA was classified into 11 genotypes. A phylogenetic tree of the 11 genotypes suggested that the genotypes observed in Brahman were a great genetic distance from other genotypes. An 18‐bp deletion–insertion variation at position 171 was found to be the result of alternative splicing. The 18‐bp deletion–insertion is located at the boundary between exon 3 and intron 3. Permanently transfected 3T3 cell lines expressing bovine <jats:italic>MX1</jats:italic> mRNA were established to analyse the antiviral potential against VSVΔG*‐G infection. Transfected cell clones expressing bovine <jats:italic>MX1</jats:italic> mRNA showed a significantly smaller number of cells infected with VSVΔG*‐G compared with the control cells. These results indicate that the bovine MX1 protein has potent antiviral activity.</jats:p>
収録刊行物
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- Animal Genetics
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Animal Genetics 35 (3), 182-187, 2004-04-23
Wiley