Preparation of marked microtubules for the assay of the polarity of microtubuie-based motors by fluorescence
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- A. A. Hyman
- Department of Pharmacology, University of California San Francisco, San Francisco, California 94143-0450, USA
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説明
<jats:title>ABSTRACT</jats:title> <jats:p>Short microtubule seeds are constructed using heavily rhodamine-labeled tubulin. Polymerisation off the ends of these seeds is initiated using a mixture of 1:10 labeled and unlabeled tubulin, so that the new polymerisation is only dimly labeled. This is done in the presence of NEM tubulin, which inhibits growth from the microtubule minus ends. The polarity-marked microtubules are fixed at a desired length by adding taxol.</jats:p>
収録刊行物
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- Journal of Cell Science
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Journal of Cell Science 1991 (Supplement_14), 125-127, 1991-01-01
The Company of Biologists