Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells
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- Xiu‐Cai Chen
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
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- Shuo‐Bin Chen
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
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- Jing Dai
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
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- Jia‐Hao Yuan
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
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- Tian‐Miao Ou
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
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- Zhi‐Shu Huang
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
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- Jia‐Heng Tan
- School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China
説明
<jats:title>Abstract</jats:title><jats:p>Because of the absence of methods for tracking RNA G‐quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G‐quadruplexes is so far limited. Herein, we report a new red‐emitting fluorescent probe, <jats:bold>QUMA‐1</jats:bold>, for the selective, continuous, and real‐time visualization of RNA G‐quadruplexes in live cells. The applications of <jats:bold>QUMA‐1</jats:bold> in several previously intractable applications, including live‐cell imaging of the dynamic folding, unfolding, and movement of RNA G‐quadruplexes and the visualization of the unwinding of RNA G‐quadruplexes by RNA helicase have been demonstrated. Notably, our real‐time results revealed the complexity of the dynamics of RNA G‐quadruplexes in live cells. We anticipate that the further application of <jats:bold>QUMA‐1</jats:bold> in combination with appropriate biological and imaging methods to explore the dynamics of RNA G‐quadruplexes will uncover more information about the biological roles of RNA G‐quadruplexes.</jats:p>
収録刊行物
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 57 (17), 4702-4706, 2018-03-13
Wiley