A Novel Fission Yeast Gene, <i>tht1</i>+, Is Required for the Fusion of Nuclear Envelopes during Karyogamy

DOI PDF 被引用文献1件
  • Yoshie Tange
    *Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan; and the ‡Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Tetsuya Horio
    *Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan; and the ‡Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Mizuki Shimanuki
    *Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan; and the ‡Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Da-Qiao Ding
    *Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan; and the ‡Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Yasushi Hiraoka
    *Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan; and the ‡Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Osami Niwa
    *Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan; and the ‡Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan

抄録

<jats:p>We have isolated a fission yeast karyogamy mutant, tht1, in which nuclear congression and the association of two spindle pole bodies occurs but the subsequent fusion of nuclear envelopes is blocked. The tht1 mutation does not prevent meiosis, so cells execute meiosis with two unfused nuclei, leading to the production of aberrant asci. The tht1+ gene was cloned and sequenced. Predicted amino acid sequence has no significant homology to previously known proteins but strongly suggests that it is a type I membrane protein. The tht1+ gene is dispensable for vegetative growth and expressed only in conjugating cells. Tht1p is a glycoprotein susceptible to endoglycosilase H digestion. Site- directed mutagenesis showed that the N-glycosylation site, as well as the COOH-terminal region of Tht1p, is essential for its function. A protease protection assay indicated that the COOH terminus is cytoplasmic. Immunocytological analysis using a HA-tagged Tht1p suggested that the protein is localized in nuclear envelopes and in the ER during karyogamy and that its levels are reduced in cells containing fused nuclei.</jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ