3D Cell Printing of Functional Skeletal Muscle Constructs Using Skeletal Muscle‐Derived Bioink
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- Yeong‐Jin Choi
- Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐guPohang Kyungbuk 790‐784 Korea
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- Taek Gyoung Kim
- Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐gu, Pohang Kyungbuk 790‐784 Korea
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- Jonghyeon Jeong
- Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐gu, Pohang Kyungbuk 790‐784 Korea
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- Hee‐Gyeong Yi
- Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐gu, Pohang Kyungbuk 790‐784 Korea
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- Ji Won Park
- Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐gu, Pohang Kyungbuk 790‐784 Korea
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- Woonbong Hwang
- Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐gu, Pohang Kyungbuk 790‐784 Korea
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- Dong‐Woo Cho
- Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) 77 Cheongam‐ro Nam‐gu, Pohang Kyungbuk 790‐784 Korea
説明
<jats:p>Engineered skeletal muscle tissues that mimic the structure and function of native muscle have been considered as an alternative strategy for the treatment of various muscular diseases and injuries. Here, it is demonstrated that 3D cell‐printing of decellularized skeletal muscle extracellular matrix (mdECM)‐based bioink facilitates the fabrication of functional skeletal muscle constructs. The cellular alignment and the shape of the tissue constructs are controlled by 3D cell‐printing technology. mdECM bioink provides the 3D cell‐printed muscle constructs with a myogenic environment that supports high viability and contractility as well as myotube formation, differentiation, and maturation. More interestingly, the preservation of agrin is confirmed in the mdECM, and significant increases in the formation of acetylcholine receptor clusters are exhibited in the 3D cell‐printed muscle constructs. In conclusion, mdECM bioink and 3D cell‐printing technology facilitate the mimicking of both the structural and functional properties of native muscle and hold great promise for producing clinically relevant engineered muscle for the treatment of muscular injuries.</jats:p>
収録刊行物
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- Advanced Healthcare Materials
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Advanced Healthcare Materials 5 (20), 2636-2645, 2016-08-16
Wiley
