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- Stefan Otte
- aDepartment of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
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- William J. Belden
- aDepartment of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
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- Matthew Heidtman
- aDepartment of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
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- Jay Liu
- aDepartment of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
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- Ole N. Jensen
- bDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense University, DK-5230 Odense M, Denmark
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- Charles Barlowe
- aDepartment of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
書誌事項
- 公開日
- 2001-01-29
- DOI
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- 10.1083/jcb.152.3.503
- 公開者
- Rockefeller University Press
この論文をさがす
説明
<jats:p>Proteins contained on purified COPII vesicles were analyzed by matrix-assisted laser desorption ionization mass spectrometry combined with database searching. We identified four known vesicle proteins (Erv14p, Bet1p, Emp24p, and Erv25p) and an additional nine species (Yip3p, Rer1p, Erp1p, Erp2p, Erv29p, Yif1p, Erv41p, Erv46p, and Emp47p) that had not been localized to ER vesicles. Using antibodies, we demonstrate that these proteins are selectively and efficiently packaged into COPII vesicles. Three of the newly identified vesicle proteins (Erv29p, Erv41p, and Erv46p) represent uncharacterized integral membrane proteins that are conserved across species. Erv41p and Erv46p were further characterized. These proteins colocalized to ER and Golgi membranes and exist in a detergent-soluble complex that was isolated by immunoprecipitation. Yeast strains lacking Erv41p and/or Erv46p are viable but display cold sensitivity. The expression levels of Erv41p and Erv46p are interdependent such that Erv46p was reduced in an erv41Δ strain, and Erv41p was not detected in an erv46Δ strain. When the erv41Δ or ev46Δ alleles were combined with other mutations in the early secretory pathway, altered growth phenotypes were observed in some of the double mutant strains. A cell-free assay that reproduces transport between the ER and Golgi indicates that deletion of the Erv41p–Erv46p complex influences the membrane fusion stage of transport.</jats:p>
収録刊行物
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- The Journal of Cell Biology
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The Journal of Cell Biology 152 (3), 503-518, 2001-01-29
Rockefeller University Press
