Coordinated control of a designed <i>trans</i>‐acting ligase ribozyme by a loop–receptor interaction
抄録
<jats:p>We previously developed a synthetic <jats:italic>cis</jats:italic>‐acting RNA ligase ribozyme with 3′–5′ joining activity termed “DSL” (designed and selected ligase). DSL was easily transformed into a <jats:italic>trans</jats:italic>‐acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a <jats:italic>trans</jats:italic>‐acting DSL, <jats:italic>t</jats:italic>DSL‐1/GUAA. <jats:italic>t</jats:italic>DSL‐1/GUAA exhibited remarkably high activity compared with the parental <jats:italic>cis</jats:italic>‐acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop–receptor interaction plays a significant role in determining the activity of the <jats:italic>trans</jats:italic>‐acting ribozyme and in its ability to perform multiple turnovers of the reaction.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 583 (17), 2819-2826, 2009-07-23
Wiley