Integrin-dependent interaction of lipid rafts with the actin cytoskeleton in activated human platelets

DOI PDF 被引用文献2件
  • Stéphane Bodin
    Inserm U.563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, 31059 Toulouse, France
  • Carine Soulet
    Inserm U.563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, 31059 Toulouse, France
  • Hélène Tronchère
    Inserm U.563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, 31059 Toulouse, France
  • Pierre Sié
    Laboratoire d'Hématologie, CHU Purpan, 31059 Toulouse, France
  • Christian Gachet
    Inserm U.311, Etablissement Français du Sang-Alsace, 10 Rue Spielman, BP 36, 67065 Strasbourg, France
  • Monique Plantavid
    Inserm U.563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, 31059 Toulouse, France
  • Bernard Payrastre
    Inserm U.563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, 31059 Toulouse, France

書誌事項

公開日
2005-02-15
DOI
  • 10.1242/jcs.01648
公開者
The Company of Biologists

この論文をさがす

説明

<jats:p>Dynamic connections between actin filaments and the plasma membrane are crucial for the regulation of blood platelet functions. Protein complexes associated with αIIbβ3 integrin-based cytoskeleton structures are known to play a role in these processes. However, mechanisms involving lateral organizations of the plasma membrane remain to be investigated. Here, we demonstrate that a large fraction of platelet lipid rafts specifically associates with the actin cytoskeleton upon activation. This association was inhibited by antagonists of fibrinogen-αIIbβ3 binding and did not occur in type I Glanzman's thrombasthenic platelets. The raft-cytoskeleton interaction is a reversible process correlating with the intensity and stability of platelet aggregation. Although only a minor fraction of αIIbβ3 was recovered in rafts upon activation, this integrin specifically upregulated the level of PtdIns(4,5)P2 in membrane microdomains and induced the recruitment of several actin-modulating proteins known to directly or indirectly interact with this lipid. Controlled disruption of rafts did not affect αIIbβ3-mediated platelet aggregation in response to high concentrations of thrombin but significantly inhibited fibrin clot retraction. We propose that rafts participate in the organization of membrane-cytoskeleton interactions where αIIbβ3-mediated tension forces apply during the late phase of platelet activation.</jats:p>

収録刊行物

被引用文献 (2)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ