Epithelial-mesenchymal transition in human gastric cancer cell lines induced by TNF-α-inducing protein of<i>Helicobacter pylori</i>

DOI Web Site PubMed 被引用文献7件 オープンアクセス
  • Tatsuro Watanabe
    Research Institute for Clinical Oncology; Saitama Cancer Center; Kitaadachi-gun Saitama Japan
  • Atsushi Takahashi
    Research Institute for Clinical Oncology; Saitama Cancer Center; Kitaadachi-gun Saitama Japan
  • Kaori Suzuki
    Research Institute for Clinical Oncology; Saitama Cancer Center; Kitaadachi-gun Saitama Japan
  • Miki Kurusu-Kanno
    Research Institute for Clinical Oncology; Saitama Cancer Center; Kitaadachi-gun Saitama Japan
  • Kensei Yamaguchi
    Saitama Cancer Center Hospital; Kitaadachi-gun Saitama Japan
  • Hirota Fujiki
    Research Institute for Clinical Oncology; Saitama Cancer Center; Kitaadachi-gun Saitama Japan
  • Masami Suganuma
    Research Institute for Clinical Oncology; Saitama Cancer Center; Kitaadachi-gun Saitama Japan

書誌事項

タイトル別名
  • Cell migration induced by Tipα of<i>H. pylori</i>
公開日
2013-11-19
権利情報
  • http://doi.wiley.com/10.1002/tdm_license_1.1
DOI
  • 10.1002/ijc.28582
公開者
Wiley

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説明

Helicobacter pylori strains produce tumor necrosis factor-α (TNF-α)-inducing protein, Tipα as a carcinogenic factor in the gastric epithelium. Tipα acts as a homodimer with 38-kDa protein, whereas del-Tipα is an inactive monomer. H. pylori isolated from gastric cancer patients secreted large amounts of Tipα, which are incorporated into gastric cancer cells by directly binding to nucleolin on the cell surface, which is a receptor of Tipα. The binding complex induces expression of TNF-α and chemokine genes, and activates NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells). To understand the mechanisms of Tipα in tumor progression, we looked at numerous effects of Tipα on human gastric cancer cell lines. Induction of cell migration and elongation was found to be mediated through the binding to surface nucleolin, which was inhibited by the nucleolin-targeted siRNAs. Tipα induced formation of filopodia in MKN-1 cells, suggesting invasive morphological changes. Tipα enhanced the phosphorylation of 11 cancer-related proteins in serine, threonine and tyrosine, indicating activation of MEK-ERK signal cascade. Although the downregulation of E-cadherin was not shown in MKN-1 cells, Tipα induced the expression of vimentin, a significant marker of the epithelial-mesenchymal transition (EMT). It is of great importance to note that Tipα reduced the Young's modulus of MKN-1 cells determined by atomic force microscopy: This shows lower cell stiffness and increased cell motility. The morphological changes induced in human gastric cancer cells by Tipα are significant phenotypes of EMT. This is the first report that Tipα is a new inducer of EMT, probably associated with tumor progression in human gastric carcinogenesis.

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