Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures
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- Marianna Prokopi
- King's British Heart Foundation Centre, King's College London, London, United Kingdom;
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- Giordano Pula
- King's British Heart Foundation Centre, King's College London, London, United Kingdom;
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- Ursula Mayr
- King's British Heart Foundation Centre, King's College London, London, United Kingdom;
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- Cécile Devue
- Paris-Cardiovascular Research Center, Inserm U970, Hôpital Européen Georges Pompidou, Université Paris-Descartes, Paris, France;
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- Joy Gallagher
- King's College Hospital, National Health Service (NHS) Foundation Trust, Blood Sciences Laboratory Services, London, United Kingdom;
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- Qingzhong Xiao
- King's British Heart Foundation Centre, King's College London, London, United Kingdom;
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- Chantal M. Boulanger
- Paris-Cardiovascular Research Center, Inserm U970, Hôpital Européen Georges Pompidou, Université Paris-Descartes, Paris, France;
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- Nigel Westwood
- Department of Haematological Medicine, King's College London, London, United Kingdom;
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- Carmen Urbich
- Molecular Cardiology, Department of Medicine III, University of Frankfurt, Frankfurt, Germany;
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- Johann Willeit
- Department of Neurology, Medical University Innsbruck, Innsbruck, Austria;
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- Marianne Steiner
- Center for Anatomy and Cell Biology, Medical University Vienna, Vienna, Austria; and
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- Johannes Breuss
- Department of Vascular Biology and Thrombosis Research, Centre for Bio-Molecular Medicine and Pharmacology, Medical University of Vienna, Vienna, Austria
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- Qingbo Xu
- King's British Heart Foundation Centre, King's College London, London, United Kingdom;
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- Stefan Kiechl
- Department of Neurology, Medical University Innsbruck, Innsbruck, Austria;
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- Manuel Mayr
- King's British Heart Foundation Centre, King's College London, London, United Kingdom;
書誌事項
- 公開日
- 2009-07-16
- DOI
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- 10.1182/blood-2009-02-205930
- 公開者
- American Society of Hematology
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>The concept of endothelial progenitor cells (EPCs) has attracted considerable interest in cardiovascular research, but despite a decade of research there are still no specific markers for EPCs and results from clinical trials remain controversial. Using liquid chromatography–tandem mass spectrometry, we analyzed the protein composition of microparticles (MPs) originating from the cell surface of EPC cultures. Our data revealed that the conventional methods for isolating mononuclear cells lead to a contamination with platelet proteins. Notably, platelets readily disintegrate into platelet MPs. These platelet MPs are taken up by the mononuclear cell population, which acquires “endothelial” characteristics (CD31, von Willebrand factor [VWF], lectin-binding), and angiogenic properties. In a large population-based study (n = 526), platelets emerged as a positive predictor for the number of colony-forming units and early outgrowth EPCs. Our study provides the first evidence that the cell type consistent with current definitions of an EPC phenotype may arise from an uptake of platelet MPs by mononuclear cells resulting in a gross misinterpretation of their cellular progeny. These findings demonstrate the advantage of using an unbiased proteomic approach to assess cellular phenotypes and advise caution in attributing the benefits in clinical trials using unselected bone marrow mononuclear cells (BMCs) to stem cell-mediated repair.</jats:p>
収録刊行物
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- Blood
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Blood 114 (3), 723-732, 2009-07-16
American Society of Hematology