Introns act post‐transcriptionally to increase expression of the <i>Arabidopsis thaliana</i> tryptophan pathway gene <i>PAT1</i>

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公開日
1997-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1046/j.1365-313x.1997.11030455.x
公開者
Wiley

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<jats:title>Summary</jats:title><jats:p>The expression of the <jats:italic>Arabidopsis thaliana PAT1</jats:italic> gene, which encodes the tryptophan biosynthetic enzyme phosphoribosylanthranilate transferase, was investigated using translational fusions of the <jats:italic>PAT1</jats:italic> promoter to the <jats:italic>GUS</jats:italic> reporter gene. Independent stably transformed <jats:italic>A. thaliana</jats:italic> lines containing a single copy of a fusion that includes the entire plastid transit peptide and the first two introns of <jats:italic>PAT1</jats:italic> had on average 30 times more GUS enzyme activity than plants transformed with a construct in which <jats:italic>GUS</jats:italic> was fused a short distance downstream of the <jats:italic>PAT1</jats:italic> start codon. Plants containing the construct without introns or leader peptide accumulated undetectable amounts of PAT1‐GUS fusion protein and mRNA, even though the transcriptional rate of both fusion constructs was comparable. Fusions containing the entire transit peptide and either of the first two introns yield as much GUS activity as constructs containing both introns, but constructs containing the transit peptide but no introns give rise to much lower levels. Therefore, introns greatly enhance the expression of <jats:italic>PAT1‐GUS</jats:italic> fusions, and they act post‐transcriptionally to increase the steady‐state level of mRNA.</jats:p>

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