Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure
書誌事項
- 公開日
- 2005-03-24
- DOI
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- 10.1186/1743-422x-2-24
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Rotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation, different G-type-specific primer sets are applied in different geographical locations. In Bangladesh, a significant proportion (36.9%) of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Four nucleotide substitutions at the G1 primer-binding site of the VP7 gene of Bangladeshi G1 rotaviruses rendered a major proportion of circulating strains untypeable using the routine primer set. Using an alternative primer set, we could identify G1 rotaviruses as the most prevalent genotype (44.8%), followed by G9 (21.7%), G2 (15.0%) and G4 (13.8%).</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Because of the natural variation in the rotaviral gene sequences, close monitoring of rotavirus genotyping methods is important.</jats:p> </jats:sec>
収録刊行物
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- Virology Journal
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Virology Journal 2 (1), 24-, 2005-03-24
Springer Science and Business Media LLC